FHV+ FCV Antigen Combo Rapid Test Cassette (Secretions), Feline Herpesvirus antigen, Feline Calicivirus antigen
INTENDED USE
FHV+FCV Antigen Combo Rapid Test Cassette (Secretions) is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Feline Herpesvirus antigen (FHV Ag) and Feline Calicivirus antigen (FCV Ag) in feline’s ocular and nasal secretions.
PRINCIPLE
FHV+FCV Antigen Combo Rapid Test Cassette is based on sandwich lateral flow immunochromatographic assay. The test cassette has two testing windows. Each testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will laterally flow on the surface of the test strip. If there is enough feline herpesvirus or feline calicivirus in the sample, a visible T line will appear.
The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can accurately indicate the presence of feline herpesvirus or feline calicivirus in the sample.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Collect feline’s ocular and nasal secretions with the swab stick. Make the swab wet sufficiently.
Note: Collect ocular secretion from upper and lower eyelids, please avoid secretions at the corner of the eye.
2. Insert the wet swab into the provided buffer tube. Agitate it to assure good sample extraction.
3. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 drops of extracted sample (approximately 120 μl) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
4. Read the result at 5 minutes. Do not interpret results after 10 minutes .

INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, no matter T line is clear or vague.
Negative: Only clear C line appears.
Invalid: No colored line appears in C zone, no matter whether T line appears.
Product Name: | FHV+ FCV Antigen Combo Rapid Test Cassette (Secretions), Feline Herpesvirus Antigen, Feline Calicivirus Antigen |
Principle: | Chromatographic Immunoassay |
Shelf Life: | 2 Years |
Specificity: | 93.10% |
Storage Temperature: | 2-30°C |
Cat No.: | VIFHC-525 |
Specimen: | Ocular And Nasal Secretions |
Sensitivity: | 90.32% |
Accuracy: | 91.67% |
Reading Time: | 5 Minutes |