CE Fluorescence Immunoassay Test Procalcitonin PCT Test Cassette

Procalcitonin (PCT) Test Cassette, With CE,Convenient To Use The Fast and Reliable PCT, Fluorescence Immunoassay Test

Applications:

The PCT Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of Procalcitonin in serum or plasma.
Description:

Procalcitonin(PCT) is a small protein that comprises 116 amino acid residues with a molecular weight of approximately 13 kDa which was first described by Moullec et al. in 1984.PCT is produced normally in Ccells of the thyroid glands. In 1993, the elevated level of PCT in patients with a system infection of bacterial origin was reported and PCT is now considered to be the main marker of disorders accompanied by systemic inflammation and sepsis. The diagnostic value of PCT is important due to the close correlation between PCTconcentration and the severity of inflammation. It was shown that “inflammatory” PCT is not produced in Ccells. Cells of neuroendocrine origin are presumably the source of PCT during inflammation.


How to use?
Allow test cassette, specimen, buffer and/or controls to equilibrate to room temperature (15-30℃) prior to testing.
1. Remove the Test Cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Place the Test Cassette on a clean and level surface.
For Serum or Plasma specimens:
Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 μL) to the specimen well (S) of the Test Cassette, and add 1 drop of buffer (approximately 40 μL), then start the timer. See illustration below.
For Venipuncture Whole Blood specimens:
Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 μL) to the specimen well (S) of the Test Cassette, and add 1 drop of buffer (approximately 40 μL), then start the timer. See illustration below.
For Fingerstick Whole Blood specimens:
To use a capillary tube: Fill the capillary tube and transfer approximately 50 μL of fingerstick whole blood specimen to the specimen well (S) of the Test Cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.
3. Wait for the colored line is appeared. The result should be read at 15minutes. Do not interpret the result after 20 minutes.

INTERPRETATION OF RESULTS
(Please refer to the illustration above)
POSITIVE:* Two distinct colored lines appear. One colored line should be in the control region (C) and another colored line should be in the test region (T).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of PCT antigen present in the specimen. Therefore, any shade of color in the test region (T) should be considered positive.
NEGATIVE: One colored line appears in the control region (C). No apparent colored line appears in the test region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.