Feline Serum Amyloid A Antigen (fSAA Ag) Test Whole Blood/Serum/Plasma VETERINARY Feline Range
Feline Serum Amyloid A (fSAA) Antigen Rapid Test Cassette is a sandwich lateral flow immunochromatographic assay for the semi quantitative detection of feline SAA antigen in whole blood and serum or plasma.
| Principle: | Chromatographic Immunoassay |
| Specimen: | WB/S/P |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 97.50% |
| Format: | Cassette |
| Reading Time: | 10 Minutes |
| Storage Temperature: | 4-30℃ |
| Sensitivity: | 98.20% |
| Accuracy: | 97.90% |
Application:
Feline Serum Amyloid A (fSAA ) Antigen Rapid Test Cassette (Whole Blood/Serum/Plasma) is a sandwich lateral flow immunochromatographic assay for the semi-quantitative detection of feline SAA antigen in whole blood and serum or plasma.
Description:
Feline Serum Amyloid A (fSAA) Antigen Rapid Test Cassette is based on lateral flow immunochromatographic assay. The test cassette has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will laterally flow on the surface of the test strip. If there is enough SAA antigen in the sample, a visible T line will appear. The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can accurately indicate the presence of feline SAA antigen in the sample.
How to use?
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Collect sample
Collect feline’s fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, serum and plasma specimens should be kept below -20°C. Whole blood should be stored at 2-8°C if the test is to be used within 2 days of collection. Do not freeze whole blood specimens.
2. Sample Dilution
Hold the small dropper vertically, draw the blood specimen or serum or plasma upto the fill line (approx.5μL) marked on the dropper as shown in illustration below, transfer the specimen into the plastic tubes with buffer.
Close the tube and shake the sample by hand forcefully for approximately 10 seconds so sample and dilution buffer mix well.
3. Place the test cassette on a clean and level surface. Hold another dropper vertically and transfer the 2 drops of Diluted specimen (approximately 80μL) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
4. Read the results in 10 minutes by comparing the T line intensity with the color card provided. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
The presence of “C” line indicates a valid result. Read the result by comparing the color intensity of the “T” line with the standard
color card provided.
1. Reference value: normal reference value＜2ug/ml;
Note:It is recommended that each laboratory establish its own reference range
2. The fSAA test can be run in between 0.5 μg/mL and 200 μg/mL;
3. fSAA concentration of 2-10ug/ml indicates the value is high or mild inflammation, while fSAA ＞10ug/ml indicates obvious inflammation.
Positive: The presence of both “C” line and zone “T” line. T line between G3 - G5 is considered as positive.
Negative: The presence of both “C” line and zone “T” line. T line at G1 or G2 is considered as negative.
Invalid:No colored line appears in C zone, regardless of T line’s appearance.
